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Molecular Strategy for Groundnut Preharvest Aflatoxin Elimination: Recent Advances and Future Prospects

Daničle Clavel1

The traditional methods used for studying host-plant resistance to A. flavus are not conducive to identifying the specific components or metabolites that directly affect aflatoxin biosynthesis. Moreover, after twenty years of research in this area, no valuable sources of resistance have been found, particularly in the groundnut germplasm. Since conventional breeding methods for controlling aflatoxin are partially effective, novel biotechnology methods are needed to develop preharvest host-plant resistance to aflatoxin accumulation. This research concerns improving knowledge on:

1.The fundamental biological mechanisms that regulate aflatoxin biosynthesis by the fungus.

2.The host-plant resistance factors to aflatoxin accumulation.

The state of knowledge on biotechnological strategies will be analyzed at the following three levels: fungal, host-plant resistance, and effects of environmental factors (drought stress).

Fungal level

Host-plant resistance

Molecular markers

Candidate gene for antifungal enzymes in groundnut

Environmental factors: drought effects on maturation process and cell membrane stability

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On-farm management of aflatoxin contamination of groundnut in West Africa.
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Crops Contaminated by A. flavus and Aflatoxin in India (Data from recent surveys).

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