AffiDYE®GelRed
A cutting‑edge fluorescent nucleic acid dye that replaces ethidium bromide in DNA/RNA gel electrophoresis offering superior sensitivity, strong thermal stability, and safer disposal.
Post‑stain (Recommended)
- Run gel (e.g. 1% agarose with standard buffer).
- Prepare 3× dye working solution (e.g., 15 µL stock + 50 mL 0.1 M NaCl).
- Soak gel for 15–30 min with gentle agitation.
- Observe under UV or blue light — no wash, no destain
Pre‑stain (In‑gel)
- Dilute stock directly into molten agarose at 1:10 000 before casting.
- Run gel with your samples.
- Visualize bands post-run—fast, one-step method.
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TAP
Post‑stain (Recommended)
- Run gel (e.g. 1% agarose with standard buffer).
- Prepare 3× dye working solution (e.g., 15 µL stock + 50 mL 0.1 M NaCl).
- Soak gel for 15–30 min with gentle agitation.
- Observe under UV or blue light — no wash, no destain
Pre‑stain (In‑gel)
- Dilute stock directly into molten agarose at 1:10 000 before casting.
- Run gel with your samples.
- Visualize bands post-run—fast, one-step method.
Components :
| Components | Volume |
| AffiDYE® GelRed | 0.5 mL |
Fig 1. and Fig 2. are AffiDYE® GelRed dye nucleic acid electrophoresis and cell membrane permeability assay (cytotoxicity assay), respectively.
